The siRNA knockdown of Src, Fyn, or Abl1 enhanced paclitaxel-mediated growth inhibition in AZD9291 solubility dmso ovarian cancer cells compared
with a control siRNA. HEY cells treated with dasatinib plus paclitaxel formed fewer colonies than did cells treated with either agent alone. Treatment of HEY xenograft-bearing mice with dasatinib plus paclitaxel inhibited tumor growth more than treatment with either agent alone (average tumor volume per mouse, dasatinib + paclitaxel vs paclitaxel: 0.28 vs 0.81 cm(3), difference = 0.53 cm(3), 95% confidence interval [CI] = 0.44 to 0.62 cm(3), P = .014); dasatinib + paclitaxel vs dasatinib: 0.28 vs 0.55 cm(3), difference = 0.27 cm(3), 95% CI = 0.21 to 0.33 cm(3), P = .035). Combined treatment induced more TUNEL-positive apoptotic cells than did either agent alone. The Selleck GNS-1480 siRNA knockdown of p27(Kip1) decreased dasatinib -and paclitaxel-induced apoptosis compared with a negative control siRNA (sub-G(1) fraction, control siRNA vs p27(Kip1) siRNA: 42.5% vs 20.1%, difference = 22.4%, 95% CI = 20.1% to 24.7%, P = .017). Studies with forced expression and siRNA knockdown of Bcl-2 and Cdk1 suggest that dasatinib-mediated induction of p27(Kip1) enhanced paclitaxel-induced apoptosis by negatively regulating Bcl-2 and Cdk1 expression.\n\nConclusion Inhibition of Src family and Abl kinases with either siRNAs or dasatinib enhances paclitaxel sensitivity of ovarian cancer cells through
p27(Kip1)-mediated suppression of Bcl-2 and Cdk1 expression.”
“To understand the response of potato to salt stress, antioxidant enzyme activities and ion content Selleckchem NVP-BSK805 were analyzed for a sensitive and a tolerant cultivar. Nodal cuttings of the tolerant cultivar, Kennebec, and the sensitive cultivar, Concord, were
exposed to media without or with 30, 60, 90 or 120 mmol/L NaCl for 4 weeks. On exposure to NaCl, the length and fresh and dry weight of both shoots and roots of Concord showed greater decrease than those of Kennebec. The decrease in shoot growth was more severe than that of the root for both cultivars. The K+ content of shoots and roots of both cultivars was reduced in a dose-dependent manner by exposure to NaCl; the Na+ content increased. Activities of ascorbate peroxidase, catalase and glutathione reductase were increased in NaCl-exposed shoots of Kennebec; the corresponding activities in NaCl-exposed shoots of Concord were decreased. Roots of both cultivars showed similar changes in the activities of these enzymes on exposure to NaCl. These studies established that enzyme activities in Concord shoots are inversely related to the NaCl concentration, whereas those in Kennebec do not show a dose dependency, which is also the case for the roots of both cultivars. Our findings suggest that an increase in activity of antioxidant enzymes, such as ascorbate peroxidase, catalase and glutathione reductase, can contribute to salt tolerance in Kennebec, a salt resistant cultivar of potato.