rhKD/APP
has activity of the Kunitz of serine protease inhibitors. It is known to inhibit proteolysis of kallikrein, plasmin and trypsin. The role of rhKD/APP has always been considered to be due to the inhibition of the exocrine pancreatic secretion in order to reduce pancreatic autodigestion and was deeply investigated. In the meanwhile rhKD/APP can inhibit cytokines and inflammation, it play a therapeutic and preventive role in AP. Methods: We use the model of ANP which is induced by injection of sodium deoxycholeaye solution into the main pancreatic duct of rats. Amylase and lipase activity were assayed and histopathological Akt inhibitor changes were observed after treatment with rhKD/APP. We observe the therapeutic and prevent effect of rhKD/APP on acute pancreatitis in rats. Results: Compared with the model group, RhKD/APP markedly inhibited Amylase and Lipase avtivity and ameliorated histopathological changes of on acute necrosis pancreatitis. Conclusion: Whereas the role of rhKD/APP in the pathogenesis of AP still need discussion. Key Word(s): 1. SCH727965 ic50 rhKD/APP; 2. pancreatitis; 3. rat; 4. pathophysiology; Presenting Author: JUNFENG XIE Additional Authors: PING XU Corresponding Author: JUNFENG XIE Affiliations: THE PEOPLE’S HOSPITAL OF GANZHOU CITY; Songjiang Branch of Affiliated First people’s Hospital of shanghai jiaotong University Objective: To study the role of NF-κB and Caspase-3 in the pathogenesis of acute pancreatitis-associated
lung injury (APALI) in rats, and the effect of pioglitazone, a ligand of peroxisome proliferator-activated receptor gamma, on these factors. Methods: A total of 54 Sprague Dawley rats were randomly and averagely divided into 3 groups, named group A, C and T. Group A and C served as SAP model and sham operation group, respectively. The rats in group T were treated with pioglitazone, an agonist of peroxisome proliferator activated receptor. The modified Li Qing-hua’s method was used to
reproduce serve acute pancreatitis (SAP) models, The histopathological changes of pulmonary tissues were examined by microscopy. The activity of myeloperoxidase (MPO) in pulmonary tissues were measured. The expression of pulmonary NF-κBp65 and Cleaved-Caspase 3 were determined by immunohistochemical staining (ABC). Results: The histological 上海皓元医药股份有限公司 examination revealed intensively inflammatory response in pulmonary tissues after SAP model was induced, but inflammatory response was alleviated in group T. The activity of MPO in group T were significantly decreased compared with group A. The activity of NF-κBp65 in group A was markedly upgraded compared with group C at all pionts (P < 0.01), which was decreased significantly in group T compared with group A at 6 h (P < 0.05). The lung expression of Cleaved-Caspase 3: The activity of Cleaved-Caspase 3 in group A and group T was markedly upgraded compared with group C at all pionts (P < 0.