Strikingly, two copies of each of the three abnormal chromosomes resulting from your t are readily identifiable inside their Figure four, together with other structural modifications noticed in TPC 1, including a del and an i. It can be possible that Basolo et al. did not recognize the cytogenetic similarities concerning these cell lines as a result of various ploidy and simply because they misinterpreted the der t as an inv. which would have a similar mor phology beneath QFQ staining. The authors also state that the two TPC one and FB2 cell lines had been utilized simultane ously in their laboratory and their experimental data about the two cell lines are identical. Since the karyotypes we determined for TPC one and FB2 are compatible using the original reports for the two cell lines, we will have to conclude that a cross contamination mishap occurred throughout the lications. Particularly, CGH had previously been utilized to TPC 1 cells with all the exact same total findings.
even if the reduced complexity in that study suggests that our TPC 1 cells acquired a number of added chromo some modifications in vitro. In accordance, a latest report by van Staveren et al. exhibits kinase inhibitor pf-562271 a G banded karyogram of TPC one that is definitely entirely compatible with our findings, whilst the corresponding karyotypic description was not pro vided. Comprehensive cytogenetic findings on B CPAP and quite just lately to the anaplastic cell lines C643, 8505C and HTH74 were also available for compari son. Using M FISH in these scientific studies allowed an extremely refined characterization of quite a few chromosomal markers of unclear origin that we also observed in our samples. We took the high resolution information and facts reported in these research into consideration when producing Table 2. Interest ingly, whereas virtually all rearranged chromosomes pre viously described for cell lines B CPAP, 8505C and attempt to set up FB2, which in truth represents a tetra ploid population of TPC 1 cells.
An additional instance of misidentity was identified when analyz ing K1 cells, which display two copies of a incredibly distinctive chromosome 1 derivative containing quite a few 9p segments. Whereas Wyllie et al. selleck chemicals LDN193189 have been the initial to report the use of K1 cells to characterize many of its molecular functions, no cytogenetic info was professional vided. Two many years later, the group that supplied Wyllie and coworkers with the K1 cell line reported its establish ment, while yet again no karyotypic information was offered. Interestingly, this group had reported in 1993 the establishment and cytogenetic characterization of a novel close to diploid thyroid cell line named GLAG 66, and the presence in the very same special chromosome 1 derivative in this cell line is quite clear from your karyotypic descrip tion and figures presented. From a cytogenetic viewpoint, the complexity of this shared rearrangement plainly confirms that K1 is a tetraploid subpopulation of your GLAG 66 cell line, which was obtained from a metas tasis of a effectively differentiated papillary thyroid carcinoma.