, State College, PA). The 95% confidence intervals BAY 73-4506 of the D10-value were also computed. To test the difference in D10-values between almond and walnut, regression lines were compared using analysis of covariance (ANCOVA) via

Minitab 15. Post-irradiation storage data were subjected to ANOVA and linear regression to test for any changes with storage time. ANOVA and Tukey’s honestly significant difference test (SAS® 9.1, SAS Institute Inc., Cary, NC) were used to evaluate the sensory data. Physical and compositional characteristics of the raw almonds and walnuts, measured before any treatment (Table 1), were consistent with published data (Agricultural Research Service, 2010 and Sze-Tao and Sathe, 2000). Corresponding EMCs for target water activities for almond and walnut (Table 2) also were consistent with published sorption isotherm data for nuts (King et al., 1983, Pahlevanzadeh and Yazdani, 2005 and Togrul and Arslan, 2007). An initial inoculation level of 8.40 ± 0.14 log CFU/g (n = 12) on almonds and 8.65 ± 0.23 log CFU/g (n = 12) on walnuts was achieved for SE PT30. For S. Tennessee, an inoculation level of 7.73 ± 0.32 (n = 12) and 7.87 ± 0.25 log CFU/g http://www.selleckchem.com/products/epz-6438.html (n = 12) was achieved for almonds and walnuts, respectively. Based on the log reductions vs. total

accumulated surface dose at the various aw levels, both SE PT30 and S. Tennessee were more resistant on walnuts than on almonds ( Fig. 1 and Fig. 2). Despite the inherent difficulty in accurately measuring surface dose on almonds and walnuts, the inactivation curves for SE PT30 on almonds and walnuts yielded statistically different slopes (α = 0.05),

based on the ANCOVA ( Table 3), reaffirming different efficacies between the two nut types. The status of water in/around microorganisms is an important factor determining the efficacy of any lethal agent. Sclareol The sorption isotherms (aw vs. equilibrium moisture content; Table 2) showed a moderately controlled adsorption process, with a 0.16 and 0.84% (d.b.) mean difference between the experimental EMC and Guggenheim–Anderson–deBoer (GAB) model predictions for almonds ( Pahlevanzadeh and Yazdani, 2005) and walnuts ( Togrul and Arslan, 2007), respectively. Sensitivity of the D10-value to water activity ( Fig. 3) is a critical design factor to consider when irradiating low water activity foods. Based on our results, SE PT30 was less resistant to irradiation at the two lowest as compared to the highest two water activities, with maximum resistance seen at 0.6–0.7 aw. It is often suggested that Salmonella becomes more resistant to lethal agents as the water activity of a food product is lowered ( Aldsworth et al., 1998, Archer et al., 1998, Carlson et al., 2005 and Shadbolt et al., 2001). However, this was only true at higher water activities ( Black and Jaczynski, 2008), as Fig. 3 illustrates that the relationship between resistance and aw is not monotonic.