Pesticides are known to differentially impact bacterial survival and growth. In a study conducted to determine the effect of pesticides on bacterial survival, Salmonella spp. were best able to survive and Listeria spp. were least able to survive in pesticide solutions, among all the bacteria tested. Bravo, the fungicide applied closest to the sampling date in this study, has been found to reduce bacterial growth, although it was less inhibitory than other products tested [34]. The addition of pesticides to the different water sources used in this study might have reduced bacterial community differentiation in the two resulting fruit

Selleckchem AZD5363 environments. The smooth texture of tomato skin may also prevent attachment and result in bacteria being washed away by rain or spray water. Although our results point to the lack of major effects of the two water sources used for pesticide applications, confirming this at the species level

for human enteric pathogens such as Salmonella, would be crucial for establishing the potential safety of surface water use for contact applications. In addition, our sampling depth analysis suggests that deeper sampling is needed for all the environments, but especially for the more diverse ws, to capture at least 90% of the community members Recent studies of analysis methodologies in bacterial diversity and metagenomics projects have revealed that small modifications or substitution of similar tools may potentially result AZD6244 supplier in significant changes in the overall biological conclusions [35–37]. In the rapidly evolving field of genomics, there

this website are few concrete standards, and the sophisticated computational protocols being developed certainly will always be sensitive to some uncertainty in the analysis parameters. To examine the sensitivity of our results to the methodology employed, we re-ran our analysis using two parallel 16S rRNA protocols from the CloVR package and found large agreement with our major results. Additionally, the 454 platform itself has ongoing issues regarding artificial Cytoskeletal Signaling inhibitor replicate generation [38] and homopolymer identification errors [39], both of which contribute to overestimation of species-level diversity in 16S rRNA-based studies. Though it is likely that our estimates of absolute species-level diversity are indeed inflated, the consistency in relative diversity differences between samples across multiple analyses is encouraging and lends support to the validity of our initial computational results and final biological and ecological conclusions. Conclusions Our research has generated the first culture-independent next-generation sequencing data set for the bacterial microbiology associated with the phyllosphere of a tomato crop under agricultural management. There are a myriad of agricultural practices that may play a role in the contamination of tomatoes by human pathogenic bacteria.