Methods: The expressions of FAF1 and FLIP proteins were detected by immunohistochemistry technique in gastric tissues of 53 patients with gastric cancer and 52 patients with gastric precancerous lesions of atrophic gastritis, and compared with those in 50 normal gastric mucosa tissues. Results: The expression rates of FAF1 protein were 84.0%,
53.85% and 28.30% in normal gastric tissue, gastric tissue of precancerous lesions and gastric cancer tissue, respectively, and there were significant differences among three groups. The expression rates of FLIP protein were 38.00% and 36.54% and 81.13% in these three groups, respectively. There were significant differences between the gastric cancer group and EGFR signaling pathway other two groups in expression rates of FLIP
protein (P < 0.01). FAF1 protein expression was related with histological differentiation of gastric carcinoma (P < 0.05). FLIP protein expression was related to the gastric cancer metastasis and TNM staging. Conclusion: 1) The expression rates of FAF1 protein in normal gastric RG7422 mw tissue, gastric precancerous lesions and gastric cancer reduced in turn. It indicated that the lack of FAF1 protein expression may play an important role in the differentiation, occurrence and development of gastric cancer. 2) The expression level of FLIP protein in gastric cancer tissues was obviously higher than those in the normal gastric tissue and gastric precancerous lesions. It indicated that the occurrence and transfer of gastric cancer might be related to
inhibition of FLIP on the apoptosis mediated by Fas/FasL. Key Word(s): 1. FAF1; 2. FLIP; 3. gastric cancer; 4. Qinghai area; Presenting Author: YANGBEI ZHU Additional Authors: JUN GAO, DUOWU ZOU Corresponding Author: DUOWU ZOU Affiliations: Changhai Hospital Objective: To determine the possible role of Vanilloid receptor 1 (TRPV1) and selleck compound cannabinoid receptors (CB1 and CB2) in the dorsal root ganglion (DRG) of rats with chronic gastroesophageal reflux disease. Methods: Male Sprague-Dawley rats were randomly divided into reflux group (R group) and control group (S group), n = 6. To established the chronic reflux model, the fundus of the rat stomach was ligated and the pyloric sphincter was restricted. The expression of TRPV1 and CB1 in T1∼T3 DRGs were analyzed by immunofluorescence and Western blot. Results: The expression of TRPV1 in DRGs relative to GAPDH was up-regulated (0.98 ± 0.01 vs 0.64 ± 0.09, p = 0.001), and the integrated optical density (IOD) of TRPV1-positive neurons was also increased (905.24 ± 134.82 vs 648.43 ± 135.13, p = 0.000). While CB1 was negative-regulated (relative to GAPDH: 0.86 ± 0.05 vs 0.96 ± 0.06, p = 0.013; IOD: 677.06 ± 123.75 vs 836.89 ± 101.00, p = 0.013). At the same time, CB2 was decreased (relative to GAPDH: 0.75 ± 0.03 vs 0.81 ± 0.