Kuffer cells are very important in the development of SAP. IL-4 and Treg promote the expression of the M2 anti-inflammatory kupffer cells, and the down -regulation of TNF-α, IL-1β and CCR7

and up-regulation of IL-10 and CD163 suggest IL-4 and Treg have therapeutic effects on SAP. Key Word(s): 1. IL-4; 2. SAP; 3. Treg; 4. Kuffer cell; Presenting Author: JIANG DAN Additional Authors: LAIMING YU Corresponding Author: LAIMING YU Affiliations: guangxi medical university Objective: To assess the effectiveness and safety of indomethacin in preventing post-endoscopic retrograde cholangiopancreatography pancreatitis (PEP). Methods: The electronic searches were conducted Pritelivir cost to retrieve Randomized controlled trials (RCTs) from the PubMed, Medline, CBM and CNKI Data. All the RCTs comparing indomethacin to placebo in prevention of PEP were identified and retrieved. Data collection and literature evaluation were performed by two reviewers independently. Review Manager 5.0 was used for statistical analysis. Results: A total of 11 RCTs involving 2718 ERCP patients were included. The Meta-analysis showed that indomethacin can reduce the incidence of PEP (OR = 0.38, 95%CI 0.28 to 0.52, P < 0.00001), and also can redue the incidence of hyperamylasemia (OR = 0.50, 95%CI 0.37 to 0.67, P < 0.00001). Conclusion: Indomethacin Olaparib mouse was safe and effective in

reducing the incidence of PEP and hyperamylasemia. Key Word(s): 1. Post-ERCP; 2. Hyperamylasemia; 3. Indomethacin; 4. Meta analysis; Presenting Author: BIN YANG Corresponding Author: BIN YANG Affiliations: Fourth Military Medical

University Objective: Few data are currently available on the effects of different lymphocyte subsets in acute pancreatitis. The aim of this study was to characterize their roles in the inflammatory cascade during acute pancreatitis. Methods: Acute pancreatitis was induced by intraperitoneal injections of cerulein in T cell-deficient (nude, n = 24) mice and wild-type control (BALB/c, selleck kinase inhibitor n = 24) mice; B cell-deficient (CBA/n, n = 24) mice and wild-type (CBA/j, n = 24) mice; and T and B cell-deficient (SCID, n = 24) mice and wild-type (C, B-17, n = 24) mice. At 6, 12, 24, and 48 h after induction with cerulein, the severity of acute pancreatitis was assessed using amylase assays, edema evaluation, and histology. The role of B lymphocyte-regulated immunity was explored using the percentage of B10-cells by flow cytometry. Inflammation was evaluated by measuring interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) levels in serum. Results: In T cell-deficient mice and T and B cell-deficient mice, serum amylase levels, pancreatic edema, and histological lesions were significantly decreased compared to controls. The expression of TNF-α and IFN-γ was reduced in mice with caerulein-induced AP, whereas they were significantly increased in B cell-deficient mice with caerulein-induced AP.