In the light of our study, all future prospective trials of the Gruppo Italiano Trapianti di Midollo (GITMO) will include the HCT-CI to stratify patients. Leukemia (2009) 23, 1131-1138; doi: 10.1038/leu.2009.1; Flavopiridol nmr published online 5 February 2009″
“Diacylglycerol kinase (DGK) is an enzyme which phosphorylates a second messenger diacylglycerol and consists of a family of isozymes that differ in terms of structural motifs, enzymological

property, and cell and tissue distribution. One of the isozymes, DGK zeta was originally shown to be expressed in various kinds of neurons under physiological conditions. However, we unexpectedly found that under pathological conditions, such as cerebral infarction, DGK zeta-immunoreactivity is detected in non-neuronal cells, although it remained to be elucidated in detail which cell types are responsible for the induced expression of DGK zeta in this setting. To further elucidate

functional implications of DGK zeta in non-neuronal cells we performed detailed immunohistochemical Stattic analysis of DGK zeta using rat brain cryoinjury model. As early as I h after cryoinjury, DGK zeta-immunoreactivity was greatly decreased in the afflicted cerebral cortex and almost disappeared in the necrotic core. On day 7 after cryoinjury, however, DGK zeta-immunoreactivity reappeared in this area. DGK zeta-immunoreactivity was clearly detected in Iba1-immunoreactive cells of an oval or ameboid shape in the VE-821 scar region, which represent activated microglia and/or macrophages. on the other hand, DGK zeta-immunoreactivity was not detected in Iba1-immunoreactive, resting microglia

of ramified and dendritic configuration in the intact cortex. Furthermore, DGK zeta-immunoreactive cells were also positive for a microglia marker GLUT5 in the scar region, but never for an astrocyte marker GFAP. Taken together, the present study reveals that DGK zeta is induced in activated microglia in brain trauma, suggesting the functional significance of DGK zeta in this process. (C) 2009 Elsevier Ireland Ltd. All rights reserved.”
“Natural killer (NK)-cell malignancies are among the most aggressive lymphoid neoplasms with very poor prognosis. We performed array comparative genomic hybridization analysis on a number of NK cell lines and primary tumors to gain better understanding of the pathogenesis and tumor biology of these malignancies. We also obtained transcriptional profiles of genes residing in these regions and compared them with normal and activated NK cells. Only 30-50% of the genes residing in the gained or deleted regions showed corresponding increased or decreased expression. However, many of the upregulated genes in regions of gain are functionally important for the proliferation and growth of the neoplastic population. Genes downregulated in regions of loss included many transcription factors or repressors, tumor suppressors or negative regulators of the cell cycle.