Deprotection with 2 M HCl produced I-123-NKJ64
with the highest radiochemical yield of 98.05+/-1.63% compared with 83.95+/-13.24% with TFA. However, the specific activity of the obtained I-123-NKJ64 was lower when measured after using 2 M HCl (0.15+/-0.23 Ci/mu mol) as the deprotecting agent in comparison to TFA (1.76+/-0.60 Ci/mu YM155 in vitro mol). Further investigation of the 2 M HCl methodology found a by-product, identified as the deprotected proto-destannylated precursor, which co-eluted with I-123-NKJ64 during the high-performance liquid chromatography (H PLC) purification.
Conclusions: The radiosynthesis of I-123-NKJ64 was achieved with good isolated radiochemical yield of 68% and a high specific activity of 1.8 Ci/mu mol. TFA was found to be the most suitable deprotecting agent, since 2 M HCl generated a by-product that could not be fully separated from I-123-NKJ64 using the HPLC methodology investigated. This study highlights the importance of HPLC purification and accurate measurement of specific activity while developing new radiosynthesis methodologies. (C) 2011 Elsevier Inc. All rights reserved.”
“Objective: Aortic surgical procedures requiring hypothermic circulatory arrest are associated with altered hemostasis and increased bleeding.
In a randomized clinical trial, we evaluated effects of thromboelastometrically guided algorithm on transfusion requirements.
Methods: Fifty-six consecutive patients (25 with acute type A dissection) undergoing
MK-4827 purchase aortic surgery with hypothermic circulatory arrest were enrolled in a randomized trial during a 6-month period. Patients were randomly allocated to treatment group (n = 27) with thromboelastometrically guided transfusion algorithm or control group (n 29) with routine transfusion practices (clinical judgment-guided transfusion followed by transfusion according to coagulation test results). Primary end point was cumulative allogeneic blood units Volasertib order (red blood cells, fresh-frozen plasma, and platelets) transfused.
Results: Transfusion of allogeneic blood was significantly reduced in the thromboelastometry group: median 9.0 units (interquartile range, 2.0-30.0 units) versus. 16.0 units (9.0-23.0 units, P = .02). Most significant decrease was in the use of fresh-frozen plasma (3.0 units, 0-12.0 units, vs 8.0 units, 4.0-18.0 units, P = .005). Postoperative blood loss (890 mL/d, 600-1250 mL/d vs 950 mL/d, 650-1400 mL/d, p = 0.5) and rate of surgical re-exploration (19% vs 24%, P = .7) were similar between groups. Thromboelastometrically guided algorithm significantly decreased need for massive perioperative transfusion (odds ratio, 0.45; 95% confidence interval, 0.2-0.9; P = .03) in multivariable logistic regression analysis.