“
“Dental caries, one of the most prevalent infectious diseases worldwide, affects approximately 80% of children

and the majority of adults. Dental caries may result in endodontic disease, leading to dental pulp necrosis, periapical inflammation and bone resorption, Go 6983 inhibitor severe pain, and tooth loss. Periapical inflammation may also increase inflammation in other parts of the body. Although many studies have attempted to develop therapies for this disease, there is still an urgent need for effective treatments. In this study, we applied a novel gene therapeutic approach using recombinant adeno-associated virus (AAV)-mediated RNAi knockdown of Cathepsin K (Ctsk) gene expression, to target osteoclasts and periapical bone resorption in a mouse model. We found that AAV-sh-Cathepsin K (AAV-sh-Ctsk) impaired osteoclast function in vivo and furthermore reduced bacterial infection-stimulated bone resorption by 88%. Reduced periapical lesion size was accompanied by decreases in mononuclear leukocyte infiltration and inflammatory cytokine expression. Our study shows that AAV-RNAi silencing of Cathepsin K in periapical tissues can significantly reduce endodontic disease development, bone destruction, and inflammation in the periapical lesion. This is the first demonstration that AAV-mediated

RNAi knockdown gene therapy may significantly reduce the severity of endodontic disease.”
“Objectives: In the field of action of disease associated with dependence the selleck compound Third Health Plan of Castilla y Leon aims specifically at promoting the adjustment of health assistance to the needs of disabled people, according to their situation.

Our objectives were:

General: To know the satisfaction level among relatives or caregivers of people who were treated according to a protocol of dental care for mentally disabled people.

Specific: To know if satisfaction is related to any sociodemographic characteristics of patients or to their pathology.

Study design: Cross-sectional study by telephone survey, set in the Primary Health Area of Salamanca.

The target population includes relatives or caregivers of mentally disabled

patients who were sent to the hospital for treatment under general anaesthesia after being attended in Primary Dental Care Units, from 1st of June/2005 BIBF 1120 cell line to 31st of May/2006.

Social and demographic variables and patients’ diseases, as well as level of satisfaction with the service, were studied through a survey.

Results: 67.4% of patients’ relatives or caregivers answered the survey, among whom 94.7% (C.I. 95%: 89-100%) were quite or very satisfied with the service in general.

Conclusion: The protocol has high acceptance despite its difficulties and it has achieved considerable improvements in several aspects of patients’ life. This level of satisfaction was not related to any sociodemographic or clinical patient characteristics.