Urinary Na/K ratios normalized gradually
with age in all patients. Growth curves of the patients were reflective of the severity of PHA and compliance with salt therapy. Functional expression studies in oocytes showed that ENaC with alpha Gly327Cys mutation, as observed in patient A. showed nearly 40% activity of the wild type ENaC. In contrast, stop mutation as in patient B reduces ENaC activity to less than 5% of the normal. Our results demonstrate distinct genotype-phenotype relationships in multi-system PHA patients. The degree of ENaC function impairment affects differently the renin-aldosterone system and urinary Na/K ratios. The differences observed are age-dependent and PHA form specific. (C) 2008 Elsevier Ltd. All rights RG-7112 reserved.”
“Aims Desmoid-type fibromatosis (DF) is a rare benign myofibroblastic neoplasm of the connective tissue that is unable to metastasize but is associated with a high local recurrence rate. Nuclear -catenin is the most commonly used histological
marker of DF; however, clinical and biological predictive markers guiding the treatment and follow-up of DF are still lacking. Normally, -catenin HDAC inhibitor is regulated by the cytoplasmic multiprotein complex of adenomatous polyposis coli (APC), axin, casein kinase 1 (CK1), and glycogen synthase kinase 3 (GSK-3); this phosphorylates and degrades -catenin, which would otherwise translocate to the nucleus. The aim of this study was to analyse the expression and localization of the -cateninprotein complex of the Wnt pathway in cells isolated from DF patients. Methods and results
We isolated cells from biopsies of DF patients, and demonstrated, by immunofluorescence and immunoblot analyses, that it is almost exclusively nuclear GSK-3 that colocalizes and interacts with -catenin. The nuclear translocation of -catenin and GSK-3 is not correlated with CTNNB1 mutations. In DF samples, the multiprotein complex is disrupted, as the cytoplasmic localization of APC and axin makes interaction with the nuclear -catenin and GSK-3 impossible. Conclusions Our data suggest that GSK-3 is an additional DF marker Torin 2 cost with an important role in the aetiopathogenesis of this entity.”
“We study how shape affects multiexciton generation rates in a semiconducting nanocrystal by considering CdSe nanorods with varying diameters and aspect ratios. The calculations employ an atomistic semiempirical pseudopotential model combined with an efficacious stochastic approach applied to systems containing up to 20 000 atoms. The effect of nanorod diameter and aspect ratio on multiexciton generation rates is analyzed in terms of the scaling of the density of trion states and the scaling of the Coulomb couplings. Both show distinct scaling from spherical nanocrystals leading to a surprising result where the multiexciton generation rates are roughly independent of the nanorod length. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.