Table 3 lists the residues from these structures used in the supe

Table 3 lists the residues from these structures used in the superpositions. Intermonomer interactions were analysed using the Protein Interfaces, Surfaces and Assemblies service (PISA) at the European Bioinformatics Institute (http://​www.​ebi.​ac.​uk/​msd-srv/​prot_​int/​pistart.​html) [69], and the Protein-Protein interface

analysis server (PROTORP) Server (http://​www.​bioinformatics.​sussex.​ac.​uk/​protorp/​ index.html) [70]. Figure preparation MAPK Inhibitor Library cost Representations of molecules were prepared using the programs PyMOL [71] and BKChem (http://​bkchem.​zirael.​org/​index.​html). The sequence alignment was visualized using Jalview [72]. The electrostatic potential of the AlrSP surface was calculated using the Adaptive Poisson-Boltzmann Solver (APBS) [73] through PyMOL. Default configurations were used for calculations. PQR files for use with APBS were generated using the PDB 2PQR Server (http://​kryptonite.​nbcr.​net/​pdb2pqr/​) [74] and the Dundee

PRODRG2 Server (http://​davapc1.​bioch.​dundee.​ac.​uk/​prodrg/​) [75]. Acknowledgements We wish to thank Eileen Murphy for her expert technical assistance, Pierre LeMagueres, Mitchell Miller, John J. Tanner and Sergey Lindeman for their expert crystallographic guidance, Michael J. Benedik and James M. Briggs for their helpful discussion HDAC inhibitor and inspiration, and MSC Rigaku, especially Kris Tesh, for data collection assistance. Funding from the National Institutes of Health, the University of Otago, and the Robert A. Welch

Foundation supported this work. References 1. Osler SW: Medicine in the Nineteenth Century. In Aequanimitas: with other addresses to medical students, nurses and and practitioners of medicine. Philadelphia: P. Blakiston’s Son & Co; 1905:217–262. 2. Jedrzejas MJ: Pneumococcal virulence factors: structure and function. Microbiol Mol Biol Rev 2001, 65:187–207.PubMedCrossRef 3. Hale KA, Isaacs D: Antibiotics in childhood pneumonia. Paediatr Respir Rev 2006, 7:145–151.PubMedCrossRef 4. World Health Organization Initiative Progesterone for Vaccine Research: Acute Respiratory Infections (Update September 2009). [http://​www.​who.​int/​vaccine_​research/​diseases/​ari/​en/​] 5. O’Brien KL, Wolfson LJ, Watt JP, Henkle E, Deloria-Knoll M, McCall N, Lee E, Mulholland K, Levine OS, Cherian T: Burden of disease caused by Streptococcus pneumoniae in children younger than 5 years: global estimates. Lancet 2009, 374:893–902.PubMedCrossRef 6. Bartlett JG, Froggatt JW: Antibiotic resistance. Arch Otolaryngol Head Neck Surg 1995, 121:392–396.PubMed 7. File TM: Community-acquired pneumonia. Lancet 2003, 362:1991–2001.PubMedCrossRef 8. Brundage JF: Interactions between influenza and bacterial respiratory pathogens: implications for LY3039478 nmr pandemic preparedness. Lancet Infect Dis 2006, 6:303–312.PubMedCrossRef 9. Klugman KP, Madhi SA: Pneumococcal vaccines and flu preparedness. Science 2007, 316:49–50.PubMedCrossRef 10.

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