James Booth for assistance with statistical analyses. Electronic supplementary material Additional file 1: Table S1: Proteins found to be differentially produced between L. Ro-3306 cell line monocytogenes parent strain 10403S and ΔBCHL. (XLSX 18 KB) Additional file 2: Table S2: Strains used in this study. (XLSX 10 KB) References 1. Chaturongakul S, Raengpradub S, Wiedmann M, Boor KJ: Modulation of stress and virulence in Listeria monocytogenes . Trends Microbiol 2008,16(8):388–396.PubMedCrossRef 2. Gray MJ, Zadoks RN, Fortes ED, Dogan B, Cai S, Chen

Y, Scott VN, Gombas HDAC inhibitor DE, Boor KJ, Wiedmann M: Listeria monocytogenes isolates from foods and humans form distinct but overlapping populations. Appl Environ Microbiol 2004,70(10):5833–5841.PubMedCrossRef PND-1186 supplier 3. Zhang C, Nietfeldt J, Zhang M, Benson AK: Functional consequences of genome evolution in Listeria monocytogenes : the lmo0423 and lmo0422 genes encode SigmaC and LstR, a lineage II-specific heat shock system. J Bacteriol 2005,187(21):7243–7253.PubMedCrossRef

4. Orsi RH, den Bakker HC, Wiedmann M: Listeria monocytogenes lineages: Genomics, evolution, ecology, and phenotypic characteristics. Int J Med Microbiol 2011,301(2):79–96.PubMedCrossRef 5. O’Byrne CP, Karatzas KA: The role of Sigma B (Sigma B) in the stress adaptations of Listeria monocytogenes : overlaps between stress adaptation and virulence. Adv Appl Microbiol 2008, 65:115–140.PubMedCrossRef 6. Oliver HF, Orsi RH, Wiedmann M, Boor KJ: Listeria monocytogenes SigmaB has a small core regulon and a conserved role in virulence but makes mafosfamide differential contributions to stress tolerance across a diverse collection of strains. Appl Environ Microbiol 2010,76(13):4216–4232.PubMedCrossRef 7. Chaturongakul S, Raengpradub S, Palmer ME, Bergholz TM, Orsi RH, Hu Y, Ollinger J, Wiedmann M, Boor KJ: Transcriptomic and phenotypic analyses identify coregulated, overlapping regulons among PrfA, CtsR, HrcA, and the alternative sigma

factors SigmaB, SigmaC, SigmaH, and SigmaL in Listeria monocytogenes . Appl Environ Microbiol 2011,77(1):187–200.PubMedCrossRef 8. Chaturongakul S, Boor KJ: RsbT and RsbV contribute to SigmaB-dependent survival under environmental, energy, and intracellular stress conditions in Listeria monocytogenes . Appl Environ Microbiol 2004,70(9):5349–5356.PubMedCrossRef 9. Wemekamp-Kamphuis HH, Wouters JA, de Leeuw PP, Hain T, Chakraborty T, Abee T: Identification of sigma factor Sigma B-controlled genes and their impact on acid stress, high hydrostatic pressure, and freeze survival in Listeria monocytogenes EGD-e. Appl Environ Microbiol 2004,70(6):3457–3466.PubMedCrossRef 10. Fraser KR, Sue D, Wiedmann M, Boor K, O’Byrne CP: Role of SigmaB in regulating the compatible solute uptake systems of Listeria monocytogenes : osmotic induction of opuC is SigmaB dependent. Appl Environ Microbiol 2003,69(4):2015–2022.PubMedCrossRef 11.