Autologous PRP is a source for obtaining growth factors especially, platelet-derived growth factor (PDGF) and transforming growth factor-? (TGF-?) that are essential for bone regeneration. The use of PRP can compound library accelerate and enhance body’s natural wound-healing mechanisms. It has the benefit to form a biological gel that may provide containment, clot stability, and function as an adhesive. CASE REPORT A 13-year-old boy reported to the Department of Pedodontics and Preventive dentistry with a chief complaint of sensitivity in the lower front teeth. Patient gave a history of trauma 3 years ago, following which he fractured his upper front tooth. There was no significant medical and/or drug history. Extraorally there was a swelling in the mandibular anterior region, with intact overlying skin and obliterating the sub-mental contour.

Intraoral examination revealed a non-vital maxillary left permanent central incisor. An intraoral swelling was seen in the mandibular arch, obliterating the anterior vestibule. On palpation, the swelling was firm and expansion of buccal and lingual cortical bone was appreciable. The lower permanent incisors were tender on percussion and gave a positive response to vitality test. They did not show any mobility and the overlying mucosa appeared normal. Regional lymph nodes were palpable and not tender. Both intraoral anterior occlusal and periapical radiographs were taken of the mandibular anterior region [Figures [Figures11 and and2].2]. Radiographic examination revealed unilocular, well defined, radiolucent areas, measuring 1.5 cm, 1 cm, and 0.

5 cm, respectively. The lesions extended from the mesial root surface of mandibular left canine to the mesial surface of contralateral canine. The involved teeth did not show loss of lamina dura and there was absence of root resorption. Figure 1 Anterior occlusal radiograph of mandibular arch showing presence of a cystic lesion Figure 2 Intraoral periapical radiograph of mandibular anterior region Clinical and radiographic findings indicated the lesion to be a solitary bone cyst. Treatment planned was surgical enucleation of the cystic lesion, followed by placement of platelet rich plasma gel into the cystic cavity. Under local anesthesia, a crevicular incision was made and a mucoperiosteal flap was raised. A round bone cutting surgical bur was used to make an opening along the margins of the cyst.

Entry into the cystic cavity showed it to be completely empty [Figure GSK-3 3]. A gentle curettage of the bony walls was done in order to induce bleeding. The thin membrane obtained from the bony cavity was sent for histological examination. Figure 3 Surgical entry into cystic cavity Preparation of autologous platelet rich plasma Prior to the surgery, 10 ml of blood was drawn intravenously from the boy and collected in a sterile plastic vaccutube coated with an anticoagulant, sodium citrate.