enterocolitica 4/O:3 strains. Yersinia enterotoxins A and B are homologues to enterotoxins found in enterotoxigenic E. coli (ETEC) and Vibrio cholerae non-O1 strains . Higher rates of diarrhoea, weight loss, and death have been detected when young rabbits were infected with a Y. enterocolitica strain that produces
heat-stable enterotoxin compared to the infection with a knock-out mutant . A majority of the Y. enterocolitica BT 1A strains possess the ystB gene  and some excrete heat-stable YstB enterotoxin at 37°C in experimental conditions corresponding to those found MK-2206 clinical trial in ileum [14, 15]. The BT 1A strains are genetically the most heterogeneous of all the Y. enterocolitica biotypes [16–19]. They belong to numerous serotypes, with at least 17 having been identified . It has been suggested that BT 1A should be separated into its own subspecies based on genetic differences on a DNA microarray against
RG7204 in vivo Y. enterocolitica ssp. enterocolitica BT 1B strain 8081 . Likewise, a number of other studies utilizing different methods have suggested that Y. enterocolitica BT 1A strains could be divided into two main clusters [16, 21–25]. However, since the studies have been conducted on different sets of strains, it is impossible to know whether all the methods would divide the strains into two clusters similarly. Recently, two genome sequences of BT 1A
strains with no evident www.selleck.co.jp/products/forskolin.html structural differences were published . Notable differences between an environmental serotype O:36 and a clinical BT 1A/O:5 strains were the presence of a Rtx toxin-like gene cluster and remnants of a P2-like prophage in the clinical BT 1A/O:5 isolate . BT 1A was the predominant biotype of Y. enterocolitica detected among Yersinia isolates from human clinical stool samples in Finland in 2006 , as also in other European countries . Of the Finnish patients with a BT 1A strain, 90% suffered from diarrhoea and abdominal pain, but only 35% had fever. Furthermore, 3% of the patients had reactive arthritis compared to 0.3% of the controls . We hypothesized that certain BT 1A strains might have a higher pathogenic potential than others. In order to study this, the clinical BT 1A isolates were investigated using multilocus sequence typing (MLST), 16S rRNA sequencing, yst-PCR, lipopolysaccharide (LPS) analysis, sensitivity to five yersiniophages and serum killing assay. MLST results were analysed with BAPS (Bayesian Analysis of Population Structure) program, genetic and phenotypic characteristics of the BT 1A strains were compared and statistical analysis was applied to assess their correlation with the symptoms of the patients. Results Genetic population structure and phylogeny In the MLST analysis, a subset of 43 Y.