In order to fulfil this aim an important effort to be made is the

In order to fulfil this aim an important effort to be made is the standardization of different formats in use to describe the same item. So, it is selleck chemicals llc relevant the adoption of thesauri

for indexing the information by concept, but also the use of permanent identificators relating to authors or institutions. Beside the DOI (Digital Object Identifier) mostly used for articles, the DAI (Digital Author Identifier) BI 6727 clinical trial and the DII (Digital Institution Identifier), already adopted by some European projects (CRIS/CERIF) may become relevant tools to mark data in a standardized way. Context metadata are the core elements of the so-called citation based networks, the privileged domain of interest and activity of the communities working in a CRIS (Current Research Information System) environment.

Momelotinib ic50 One particular type of CRIS standard for information systems is the CERIF (Common European Research Information Format) standard, proposed by the European Union and developed and maintained by euroCRIS. This relevant perspective for the future of repository technology was recently debated at international level during a Workshop organized by the Institute for Research on Population and Social Policies of the National Research Council (CNR), in Rome [26]. Turning to the ongoing Italian initiatives with metadata storage and supply in the biomedical field, the experience gained by the Istituto Superiore di Sanità is worth to be mentioned. In 2004 the ISS launched a project aimed at creating a digital archive compliant with the aims of the Open Archives Initiative. In 2006 the ISS built up its own repository, DSpace ISS based on the DSpace platform [27]. The primary object was to provide both data and services regarding research material produced by the ISS most research staff. DSpace is an OAI compliant open-source software released by MIT (Massachusetts

Institute of Technology, US) for archiving e-prints and other kinds of academic content. It preserves and enables easy and open access to all types of digital content including text, images and data sets. The primary goals to be achieved were to store digital information and index it by assigning descriptive metadata in order to keep research material accessible and to preserve content in a safe archive, according to an internal policy (Institutional Policy for Open Access to Scientific Publications) available from the home page of DSpace ISS website. Content retrieval based on the adoption of MeSH terms in the indexing of DSpace ISS items has also featured the repository from the very beginning [28].

9% NaCl as collecting fluid (exact volume determined for each sam

9% NaCl as collecting fluid (exact volume determined for each sample). The samples were frozen at -80 °C and shipped to Zürich on dry ice for further analyses. There, freshly defrosted samples were vortexted for 1 min, sonicated for 5 s, aliquoted and assessed by FISH. Aliquots were also grown at 37 °C anaerobically and in 10% CO2 on LBS agar (Becton Dickinson) with the aim to isolate and type representative strains by partial 16S ARS-1620 concentration rDNA sequencing. Demineralization of discs was determined by quantitative

light-induced fluorescence as described [29]. Preparation of multi-well slides for FISH Overnight cultures of lactobacilli (LBS broth) were washed in 0.9% NaCl, diluted in coating buffer [30], spotted on 18- or 24-well

slides (Cel-Line Associates), air-dried, and fixed in 4% paraformaldehyde/PBS (20 min, 4 °C). Analogously, in situ grown biofilm samples, supragingival plaque samples and tongue scrapings were vortexed at maximum speed for 60 s, diluted in coating buffer and coated to 18- or 24-well slides as described [30]. To improve cell wall permeability PX-478 order each well selected for FISH of lactobacilli was treated individually at room temperature first for 5 min with 9 μl of lysozyme (1 mg ml-1; Sigma-Aldrich L-7651) and achromopeptidase (1 mg ml-1; Sigma-Aldrich A-7550) TGF-beta inhibitor in Tris-HCl (pH 7.5) with 5 mM EDTA, and then for 30 min with 9 μ l of lipase (Sigma-Aldrich L-1754; at 25 mg ml-1 in water the lipase suspension was centrifuged for 5 min at 16’000 × g after which the supernatant was used). Thereafter, to limit unspecific FISH probe binding all wells were covered for 30 min at 37 °C with 9 μ l of PBS containing Denhardt’s solution (Fluka 30915; diluted 1:50) in the presence of protectRNA RNase inhibitor (Sigma-Aldrich R-7397; diluted 1:500) [15, 16, 26, 27]. At the end of the respective incubation periods the solutions were carefully aspirated and the slides briefly washed

in wash-buffer (0.9% NaCl, 0.05% Tween 20, 0.01% NaN3), dipped in water, and air-dried. All solutions were made with water of nano-pure quality. Fluorescent in situ hybridization The 16S rRNA targeted oligonucleotide probes used in this study are S6 Kinase inhibitor listed in Table 1. Custom-synthesized by Microsynth, they were labeled at 5′-end with Cy3 or 6-FAM, or in some cases at both ends with 6-FAM. Probes marked by “”L-”" in front of the probe name, contain one or two LNA to improve in situ hybridization efficiency [16]. Probes were designed as described previously [30] using the ARB software [31] with the SILVA rRNA database [32, 33] and additional rRNA sequence information from ‘The Ribosomal Data Base Project II’ [34, 35] and the ‘National Center for Biotechnology Information’ [36].

In the present study, we further examined the tumor-suppressing f

In the present study, we further examined the tumor-suppressing function of ECRG4 gene, in terms of cell migration and invasion, 10058-F4 in vitro and explored possible molecular mechanism in ESCC. Materials and methods Construction of eukaryotic expression vector and stable transfection The coding region of ECRG4 cDNA was subcloned into constitutive mammalian expression vector pcDNA3.1 (Invitrogen). The cDNA was then fully sequenced to ensure that no mutation was introduced during

the PCR amplification. The resulting plasmid construct was named pcDNA3.1-ECRG4. The human esophageal squamous cell line EC9706 was established and studied by Han et al [9]. EC9706 cells were seeded in 6-cm dishes at 5×105 cells/dish and transfected with pcDNA3.1-ECRG4 PF 01367338 and pcDNA3.1 using lipofectamine™2000 (Invitrogen), according to the manufacturer’s protocol. After culturing in medium containing 400 μg/ml of Alvocidib mouse geneticin (Invitrogen) for 3 weeks, individual clones were isolated. Clones that expressed the ECRG4 cDNA coding region were maintained in medium containing 200 μg/ml of geneticin and used for further experiments. Cell proliferation assay EC9706 cells (pcDNA3.1 and pcDNA3.1-ECRGR4) were seeded into 96-well plates (1.5 × 103 cells/well). After culturing for various durations, cell proliferation was evaluated by thiazolyl blue tetrazolium bromide (MTT) assay, according to the manufacturer’s protocol (Sigma-Aldrich

Co., St. Louis, MO, USA). In brief, 10 μl MTT solution (5 mg/ml) was added to each well, then the cells were cultured for another 4 hours at 37°C, and 100 μl DMSO was added to each well and mix vigorously to solubilize colored crystals produced within the living cells. The absorbance at 570 nm was measured by using a multi-well scanning spectrophotometer Victor 3. In vitro cell migration and invasion assay Selleckchem Ibrutinib Cells growing in the log phase were treated with trypsin and re-suspended as single-cell solutions. A total of 1 × 105 cells in 0.5 ml of serum-free RPMI 1640 medium were seeded on a 8 μm-pore polycarbonate membrane Boyden chambers insert in a transwell apparatus(Costar,

Cambridge, MA), either coated with or without Matrigel(BD Biosciences, San Jose, CA). 600 μl RPMI1640 containing 20% FBS was added to the lower chamber. After the cells were incubated for 12-24 hours at 37°C in a 5% CO2 incubator, cells on the top surface of the insert were removed by wiping with a cotton swab. Cells that migrated to the bottom surface of the insert were fixed in 100% methanol for 2 minutes, stained in 0.5% crystal violet for 2 min, rinsed in PBS and then subjected to microscopic inspection (×200). Values for invasion and migration were obtained by counting five fields per membrane and represent the average of three independent experiments. Cell adhesion assay Cells were plated on 100 ng/μl Matrigel-coated 96-well plates at a density of 5 × 104 per well.

PloS one 2013,8(7):e69240 PubMedCentralPubMedCrossRef


PloS one 2013,8(7):e69240.PubMedCentralPubMedCrossRef

22. Li J, Cao B, Liu X, Fu X, Xiong Z, Chen L, Sartor O, Dong Y, Zhang H: Berberine suppresses androgen receptor signaling in prostate cancer. Mol Canc Ther 2011,10(8):1346–1356.CrossRef 23. Park KS, Kim JB, Bae J, Park SY, Jee HG, Lee KE, Youn YK: Berberine inhibited the growth of thyroid cancer cell lines 8505C and TPC1. Yonsei Med J 2012,53(2):346–351.PubMedCentralPubMedCrossRef 24. Mahata S, Bharti AC, Shukla S, Tyagi A, Husain SA, Das BC: Berberine modulates AP-1 activity to suppress HPV transcription and downstream signaling to induce growth arrest and apoptosis in cervical cancer cells. Mol Cancer 2011, 10:39.PubMedCentralPubMedCrossRef 25. Hui L, Bakiri L, Stepniak E, Wagner EF: p38alpha: a suppressor of cell proliferation and tumorigenesis. Cell Crenolanib ic50 Cycle 2007,6(20):2429–2433.PubMedCrossRef 26. Lee HJ, Auh QS, Lee YM, Kang SK, Chang SW, Lee DS, Kim YC, Kim EC: Growth inhibition and c-Met inhibitor apoptosis-inducing effects of Cudraflavone B in human oral cancer cells via MAPK, NF-kappaB, and SIRT1 signaling pathway. Planta Med 2013,79(14):1298–1306.PubMedCrossRef 27. Park HS, Hwang HJ, Kim GY, Cha HJ, Kim WJ, Kim

Selleck BAY 73-4506 ND, Yoo YH, Choi YH: Induction of apoptosis by fucoidan in human leukemia U937 cells through activation of p38 MAPK and modulation of Bcl-2 family. Mar Drugs 2013,11(7):2347–2364.PubMedCentralPubMedCrossRef 28. Cok A, Plaisier C, Salie MJ, Oram DS, Chenge J, Louters LL: Berberine acutely activates the glucose transport activity of GLUT1. Biochimie 2011,93(7):1187–1192.PubMedCentralPubMedCrossRef 29. Burgeiro A, Gajate C, el Dakir H, Villa-Pulgarin JA, Oliveira PJ, Mollinedo F: Involvement of mitochondrial and B-RAF/ERK signaling pathways in berberine-induced apoptosis in human melanoma cells. Anti-cancer drugs 2011,22(6):507–518.PubMedCrossRef FAD 30. Cheng B, Song J, Zou Y, Wang Q, Lei Y, Zhu C, Hu C: Responses of vascular smooth muscle cells to estrogen are dependent on balance between ERK and p38 MAPK pathway activities. Int J Cardiol 2009,134(3):356–365.PubMedCrossRef

31. Finch AR, Caunt CJ, Perrett RM, Tsaneva-Atanasova K, McArdle CA: Dual specificity phosphatases 10 and 16 are positive regulators of EGF-stimulated ERK activity: indirect regulation of ERK signals by JNK/p38 selective MAPK phosphatases. Cell Signal 2012,24(5):1002–1011.PubMedCentralPubMedCrossRef 32. Li J, Gu L, Zhang H, Liu T, Tian D, Zhou M, Zhou S: Berberine represses DAXX gene transcription and induces cancer cell apoptosis. Lab Invest 2013,93(3):354–364.PubMedCentralPubMedCrossRef 33. Halacli SO, Canpinar H, Cimen E, Sunguroglu A: Effects of gamma irradiation on cell cycle, apoptosis and telomerase activity in p53 wild-type and deficient HCT116 colon cancer cell lines. Oncol Lett 2013,6(3):807–810.PubMedCentralPubMed 34.

Br J Haematol 2004,125(6):749–755 PubMed 160 Eisenbarth GS: Upda

Br J Haematol 2004,125(6):749–755.PubMed 160. Eisenbarth GS: Update in type 1 diabetes. J Clin Endocrinol Metab 2007,92(7):2403–2407.PubMed 161. Aiello LP, Gardner TW, King GL, Blankenship G, Cavallerano JD, Ferris FL, Klein R: Diabetic retinopathy. Diabetes Care 1998,21(1):143–156.PubMed 162. Sima AA, Zhang W, Grunberger G: Type 1 diabetic neuropathy and C-peptide. Exp Diabesity Res 2004,5(1):65–77.PubMed 163. Ingberg

CM, Palmer M, Schvarcz E, Aman J: Prevalence of urinary tract symptoms in long-standing type 1 diabetes mellitus. Diabetes Metab 1998,24(4):351–354.PubMed 164. Couri CE, Oliveira MC, Stracieri AB, Moraes DA, Pieroni F, Barros GM, Madeira MI, Malmegrim KC, Foss-Freitas MC, Simoes BP, et al.: C-peptide levels and insulin independence following autologous nonmyeloablative hematopoietic stem cell transplantation in newly diagnosed type 1 diabetes mellitus. JAMA 2009,301(15):1573–1579.PubMed 165. Snarski E, Torosian T, Paluszewska 4SC-202 in vitro M, Urbanowska E, Milczarczyk A, Jedynasty K, Franek E, Jedrzejczak WW: Alleviation of exogenous insulin requirement in type

1 diabetes mellitus after immunoablation APR-246 molecular weight and transplantation of autologous hematopoietic stem cells. Pol Arch Med Wewn 2009,119(6):422–426.PubMed 166. Trivedi HL, Vanikar AV, Thakker U, Firoze A, Dave SD, Patel CN, Patel JV, Bhargava AB, Shankar V: Human adipose tissue-derived mesenchymal stem cells combined with hematopoietic stem cell transplantation synthesize insulin. Transplant Proc 2008,40(4):1135–1139.PubMed 167. Wijesekera LC, Leigh PN: Amyotrophic lateral sclerosis. Orphanet ID-8 J Rare Dis 2009, 4:3.PubMed 168. Janson CG, Ramesh TM, During MJ, Leone P, Heywood J: Human intrathecal transplantation of peripheral blood stem cells in amyotrophic lateral sclerosis. J Hematother Stem Cell Res 2001,10(6):913–915.PubMed 169. Mazzini L, Ferrero I, Luparello V, Rustichelli D, Gunetti M, Mareschi K, Testa L, Stecco A, Tarletti R, Miglioretti M, et al.: Mesenchymal stem cell transplantation

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2011; Lamichhaney et al 2012; Limborg et al 2012; DeFaveri et a

2011; Lamichhaney et al. 2012; Limborg et al. 2012; DeFaveri et al. 2013); ocean connectivity has been correlated with genetic divergence in herring (Teacher

et al. 2013) as has temperature for herring and three-spined stickleback (Limborg et al. 2012; DeFaveri et al. 2013). Additional factors that have been demonstrated to affect genetic structure include larval development and dispersal (Kyle and Boulding 2000). For example, the free-floating larval stage in Atlantic herring and a later pelagic life stage mediate potential for long distance dispersal and is a likely explanation for the lack of genetic structuring for herring within the Baltic Sea shown here, as well as in previous studies using neutral genetic markers (Bekkevold et al. 2005; Jørgensen et al. 2005). Genetic divergence among herring populations has indeed been shown to be affected more by ocean selleck chemicals llc currents than geographic

distance (Teacher et al. 2013). Ocean currents are more likely to affect species with freefloating life stages, such as herring, or bladderwrack, for which dispersal of eggs are limited, but detached adults have the potential for dispersal by means of rafting (Tatarenkov et al. 2007). Species with stationary development on the other hand, such as European whitefish and Northern pike, which are both associated with freshwater spawning, are likely to have more limited dispersal. The observed pattern of LY333531 isolation by distance found in whitefish and pike in the present study as well as previous studies (Laikre et al. 2005b; Olsson et al. 2012a) is consistent with such limited dispersal and suggests that migration predominantly takes place between geographically proximate populations. It should be noted that recent studies have detected isolation by distance also in herring (Teacher et al. 2013) and three-spined and nine-spined stickleback (DeFaveri et al. 2012). Those studies included

either larger sample sizes and/or more genetic markers than examined here, however, and may thus have been characterized by higher statistical power for detection of isolation by distance. Other factors potentially affecting genetic diversity in the Baltic Sea include postglacial colonization of the area by different phylogenetic lineages. Nine-spined stickleback in the Baltic Sea has been shown to consist of one western and one eastern lineage meeting roughly at the entrance of the Baltic Sea (Shikano et al. 2010; Teacher et al. 2011), as previously also shown for cod (Nielsen et al. 2003) and the bivalve Macoma balthica (Luttikhuizen et al. 2012). A more extreme example of transition zones is represented by the blue Quizartinib research buy mussel, where the species M. trossulus, native to the Baltic Sea is hybridized with M. edulis (Riginos and Cunningham 2005).

During silica synthesis by sol–gel process under certain conditio

During silica synthesis by sol–gel process under certain conditions like restriction of gel growth, silica gets precipitated. In such preparation, the steps buy ARRY-438162 involved are coagulation and precipitation from silica solution. In the present investigation, we have focused our effort on preparing stable nanosilica from selleck screening library sodium silicate which was synthesized from Vietnamese rice husk using the sol–gel technique. Main text Materials Rice husk from the

natural rice source of Mekong Delta, Vietnam, was used. Sodium hydroxide, cetyltrimethylammonium bromide (CTAB), cetyl amine (CA), polyethylene glycol (PEG, 10,000), Arkopal, cethyl ammonium chloride (CAC), Aliquat 336, alkyl dimethyl benzyl ammonium chloride (ADBAC), cetylpyridiniumbromide (CPB), and cetyltrimethylammonium

chloride (CTAC) were purchased SRT2104 clinical trial from Merck (Darmstadt, Germany) and used as surfactant agents. Chlorhydric acid, sulfuric acid, and n-butanol were all purchased from Xilong (Guangzhou, China). Experimental procedure Pretreatment of the RHA The pretreatment of the RHA consisted of acid and thermal treatments. After treating the RH with 10% HCl and 30 wt.% sulfuric acid solution, the material was burned in a muffle furnace at 600°C for 4 h to remove all incorporated hydrocarbons. An acid washing step was used to remove the small quantities of minerals prior to silica extraction from RHA in the following manner. The calcinated RHA (10

g) was acid-leached with 10% HCl and afterwards 30 wt.% sulfuric acid solution at 100°C for 2 h in a Pyrex three-neck round-bottom flask equipped with a reflux condenser in a hemispherical heating mantle. Then, the slurry was filtered and washed with distilled water for several times until the pH value equaled 7. Preparation of sodium silicate solution Sodium hydroxide solution (3.5 mol/L) was added to the pretreated RHA and boiled for 5 h in a Pyrex three-neck round-bottom flask equipped with a reflux condenser in a hemispherical heating mantle to dissolve the silica and to produce a sodium silicate solution. The solution was filtered and washed with boiling distilled water. The final solid sample was cooled to room temperature. Synthesis of silica Methane monooxygenase nanoparticles Surfactant (2.0 wt.%) was dissolved in the water/butanol (1:1) solvent. Subsequently, RHA-derived sodium silicate was slowly added into the CTAB/water/butanol solution, and the mixture was stirred at 60°C. Then, 0.5 mol/L sulfuric acid solution was added gradually into the suspension in order to initiate the hydrolysis-condensation reaction at pH ~ 4. The resulting gel mixture was aged at 60°C for 8 h. Then, 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 wt.% of CTAB were dissolved in the water/butanol solvent with 1:1 ratio. Subsequently, RHA-derived sodium silicate was slowly added to the CTAB water/butanol solution that was being stirred at 60°C. Then, 0.

Table 3 lists the residues from these structures used in the supe

Table 3 lists the residues from these structures used in the superpositions. Intermonomer interactions were analysed using the Protein Interfaces, Surfaces and Assemblies service (PISA) at the European Bioinformatics Institute (http://​www.​ebi.​ac.​uk/​msd-srv/​prot_​int/​pistart.​html) [69], and the Protein-Protein interface

analysis server (PROTORP) Server (http://​www.​bioinformatics.​sussex.​ac.​uk/​protorp/​ index.html) [70]. Figure preparation MAPK Inhibitor Library cost Representations of molecules were prepared using the programs PyMOL [71] and BKChem (http://​bkchem.​zirael.​org/​index.​html). The sequence alignment was visualized using Jalview [72]. The electrostatic potential of the AlrSP surface was calculated using the Adaptive Poisson-Boltzmann Solver (APBS) [73] through PyMOL. Default configurations were used for calculations. PQR files for use with APBS were generated using the PDB 2PQR Server (http://​kryptonite.​nbcr.​net/​pdb2pqr/​) [74] and the Dundee

PRODRG2 Server (http://​davapc1.​bioch.​dundee.​ac.​uk/​prodrg/​) [75]. Acknowledgements We wish to thank Eileen Murphy for her expert technical assistance, Pierre LeMagueres, Mitchell Miller, John J. Tanner and Sergey Lindeman for their expert crystallographic guidance, Michael J. Benedik and James M. Briggs for their helpful discussion HDAC inhibitor and inspiration, and MSC Rigaku, especially Kris Tesh, for data collection assistance. Funding from the National Institutes of Health, the University of Otago, and the Robert A. Welch

Foundation supported this work. References 1. Osler SW: Medicine in the Nineteenth Century. In Aequanimitas: with other addresses to medical students, nurses and and practitioners of medicine. Philadelphia: P. Blakiston’s Son & Co; 1905:217–262. 2. Jedrzejas MJ: Pneumococcal virulence factors: structure and function. Microbiol Mol Biol Rev 2001, 65:187–207.PubMedCrossRef 3. Hale KA, Isaacs D: Antibiotics in childhood pneumonia. Paediatr Respir Rev 2006, 7:145–151.PubMedCrossRef 4. World Health Organization Initiative Progesterone for Vaccine Research: Acute Respiratory Infections (Update September 2009). [http://​www.​who.​int/​vaccine_​research/​diseases/​ari/​en/​] 5. O’Brien KL, Wolfson LJ, Watt JP, Henkle E, Deloria-Knoll M, McCall N, Lee E, Mulholland K, Levine OS, Cherian T: Burden of disease caused by Streptococcus pneumoniae in children younger than 5 years: global estimates. Lancet 2009, 374:893–902.PubMedCrossRef 6. Bartlett JG, Froggatt JW: Antibiotic resistance. Arch Otolaryngol Head Neck Surg 1995, 121:392–396.PubMed 7. File TM: Community-acquired pneumonia. Lancet 2003, 362:1991–2001.PubMedCrossRef 8. Brundage JF: Interactions between influenza and bacterial respiratory pathogens: implications for LY3039478 nmr pandemic preparedness. Lancet Infect Dis 2006, 6:303–312.PubMedCrossRef 9. Klugman KP, Madhi SA: Pneumococcal vaccines and flu preparedness. Science 2007, 316:49–50.PubMedCrossRef 10.

Alkalinizing agents including sodium bicarbonate


Alkalinizing agents including sodium bicarbonate

(NaHCO3) have been proposed as ergogenic aids for their potential effects on providing enhanced extracellular buffer capacity, leading to the elevated proton (H+) efflux from the contracting musculature [9, 10]. The increased intramuscular [H+] during exercise has been considered as one of the major causes of muscle fatigue [11]. It has been suggested that H+ accumulation would inhibit the enzymes involved in oxidative phosphorylation and glycolysis. It would also reduce Ca2+ binding to troponin C and inhibit the sarcoplasmic reticulum enzyme Ca2+-ATPase [11, 12]. Indeed, previous studies generally agreed that NaHCO3 supplementation was beneficial for the performance in a single bout of high-intensity exercise lasting 1-7 min [13, 14], and intermittent short-term high-intensity exercise [15–17]. It has mTOR inhibitor also been shown that NaHCO3 supplementation increased the total work output during a 1-hr competitive cycling [18]. Furthermore, NaHCO3 supplementation could improve total power output in a 30 min high-intensity intermittent

cycling exercise representative MLN8237 datasheet of various ball games [19]. Nevertheless, several studies failed to find ergogenic effect of NaHCO3 supplementation on exhaustive short-term cycling [20] or resistance exercise [21]. Recently, the potential role of NaHCO3 supplementation in alleviating the exercise-induced impairment Thymidylate synthase in the neural functions has been proposed. NaHCO3 supplementation has been shown to increase muscle fiber conduction velocity and reduce force decline in sustained maximal contraction after a 50-min submaximal cycling [22]. With the potential role of NaHCO3 in preserving the neural functions after prolonged exercise, we hypothesized that NaHCO3 supplementation may prevent the fatigue-induced decline in skilled tennis performance. The aim of

this study was to investigate the effect of NaHCO3 supplementation on skilled tennis performance after a simulated match. Materials and methods Participants Nine male Division I buy YH25448 College tennis players (age 21.8 ± 2.4 years; height 1.73 ± 0.07 m) were recruited. All participants have competed in the national level. All participants were given their written informed consent. The study protocol was approved by the Human Subject Committee of National Taiwan College of Physical Education. Experimental design This study used a randomized cross-over, placebo-controlled, double-blind design. Each participant completed 2 experimental trials, bicarbonate and placebo, in a randomized order. The 2 trials were separated by 1 week. The schedule of dietary supplementation, exercise test, and blood sampling is shown in Figure 1. All trials were performed in the same outdoor tennis court with a hard surface. The temperature at the start of the exercise was 34.5 ± 3.2°C and 34.4 ± 3.4°C in the placebo and bicarbonate trial, respectively. The relative humidity was 47.

CrossRef 7 Jung CU, Yamada H, Kawasaki M, Tokura Y: Magnetic ani

CrossRef 7. Jung CU, Yamada H, Kawasaki M, Tokura Y: Magnetic anisotropy control of SrRuO 3 films by tunable epitaxial strain. Appl Phys Lett 2004, 84:2590–2592.CrossRef 8. Lee BW, Jung CU: Modification of magnetic properties through the control of growth orientation and epitaxial strain in SrRuO 3 thin films. Appl Phys Lett 2010, 96:102507.CrossRef 9. Lee BW, Jung CU: Coherent growth behavior of an orthorhombic (Ca, Sr)SnO 3 thin films on a cubic SrTiO 3 (110) substrate. J Korean Phys Soc 2012, 61:795–798.CrossRef 10. Tokura Y, Tomioka Y: Colossal magnetoresistive manganites. J Magn Magn Mater 1999, 200:1.CrossRef 11. Salamon MB, Jaime M: The physics of manganites: structure

and transport. Rev Mod Phys 2001, 73:583.CrossRef 12. Imada M, Fujimori A, Tokura Y: Metal-insulator transition. see more Rev Mod Epacadostat purchase Phys 1998, 70:1039.CrossRef 13. Kim DH, Aimon NM, Bi L, Florez JM, Dionne GF, Ross CA: Magnetostriction in epitaxial SrTi 1- x Fe x O 3- δ perovskite films with x = 0.13 and 0.35. J Phys Condens Matter 2013, 25:026002.CrossRef 14. Lee BW, Jung CU, Kawasaki M, Tokura Y: Tuning of magnetism in SrRuO 3 thin films on SrTiO

3 (001) substrate by control of the twin and strain amount in the buffer layer. J Appl Phys 2008, 104:103909.CrossRef 15. Kim NG, Kumar N, Park YA, Hur N, Jung CU, Jung JH: Application of magnetic fields for a low temperature growth of high-quality SrRuO 3 thin films. J Phys D Appl Phys 2008, 41:125005.CrossRef 16. Sekigughi S, Fujimoto M, Nomura M, Cho S-B, Tanaka J, Nishihara T, Kang

M-G, Park H-H: Atomic force microscopy observation of SrTiO 3 polar surface. Solid State Ion 1998, 108:73–79.CrossRef 17. Chang J, Park Y-S, Kim S-K: Atomically flat single-terminated SrTiO 3 (111) surface. Appl Phys Lett 2008, 92:152910.CrossRef 18. Biswas A, Rossen PB, Yang C-H, Siemons W, Jung M-H, Yang IK, Ramesh R, Jeong YH: Universal Ti-rich termination of atomically flat SrTiO 3 (001), (110), (111) surfaces. Appl Phys Lett 2011, 98:051904.CrossRef 19. Connell JG, Isaac BJ, Ekanayake GB, Strachan DR, Seo SSA: Preparation of atomically flat SrTiO 3 surfaces using a deionized-water leaching and thermal annealing procedure. Appl Phys Lett 2012, 101:251607.CrossRef 20. Vailionis A, Siemons W, Koster Chloroambucil G: Strained-induced single-domain growth of epitaxial SrRuO 3 layers on SrTiO 3 : a high-temperature X-ray diffraction study. Appl Phys Lett 2007, 91:071907.CrossRef 21. Choi KJ, Baek SH, Jang HW, Belenky LJ, Lyubchenko M, Eom C-B: Phase-transition temperature of strained single-crystal SrRuO 3 thin films. Adv Mater 2010, 22:759–762.CrossRef 22. Grutter A, Wong F, Arenholz E, Liberati M, Vailionis A, Suzuki Y: Enhanced magnetism in epitaxial SrRuO 3 thin films. Appl Phys Lett 2010, 96:082509.CrossRef 23. Hong W, Lee HN, Yoon M, Christen HM, ABT-737 manufacturer Lowndes DH, Suo Z, Zhang Z: Persistent step-flow growth of strained films on vicinal substrates. Phys Rev Lett 2005, 95:095501.CrossRef 24.