By microarray analysis of vector-infected cells, we observed that individual members of Raf inhibitor this vector population expressed unique PC12 cDNA-derived mRNA, demonstrating the power of this system to transfer and express a variety of gene activities. We discuss the potential utility of this and similarly derived expression libraries for genome-wide approaches to identify cellular functions that participate in complex host-pathogen interactions or processes related to disease and to cell growth and development.”
“Earlier studies reported exacerbated locomotor response to stress and tranylcypromine in beta 2 nicotinic acetylcholine receptor (nAChR) knockout (KO) mice. This
study aimed to further assess the role of beta 2 and coexpressed nAChR subunits in the brain (alpha 4,
alpha 6 and alpha 7) to control monoamine-mediated locomotor response, that is, response to novelty, saline, nicotine with tranylcypromine pretreatment, cocaine, d-amphetamine check details and morphine treatments. Results show that beta 2 KO mice were hyperreactive to novelty, cocaine and morphine. In contrast, alpha 7 KO mice were hyporeactive to tranylcypromine and cocaine. These results suggest that endogenous nAChR stimulation may exert a tonic control on monoamine-mediated locomotor responses. beta 2 and alpha 7-containing nAChR may contribute, respectively, to the inhibitory and permissive pathways of this tonic control. NeuroReport 21:1085-1089 (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.”
“The alphavirus Semliki Forest virus (SFV) and its derived
vectors induce apoptosis in mammalian cells. Here, we show that apoptosis is associated with the loss of mitochondrial membrane potential followed by the activation of caspase-3, caspase-8, and caspase-9. Cell death can be partially suppressed by treatment with the GSK3326595 price pan-caspase inhibitor zVAD-fmk. To determine the role of SFV structural proteins in cell death, the temporal course of cell death was compared in cells infected with SFV and cells infected with SFV virus replicon particles (VRPs) lacking some or all of the virus structural genes. In the absence of virus structural proteins, cell death was delayed. The endoplasmic reticulum (ER) stress response, as determined by the splicing of X-box binding protein 1 (XBP1) transcripts and the activation of caspase-12, was activated in virus-infected cells but not in VRP (SFV lacking structural genes)-infected cells. The C/EBP-homologous protein (CHOP) was upregulated by both virus and VRP infections. The virus envelope proteins but not the virus capsid protein triggered ER stress. These results demonstrate that in NIH 3T3 cells, SFV envelope glycoproteins trigger the unfolded protein response of the ER and accelerate apoptotic cell death initiated by virus replicase activity.”
“We have earlier shown that collagen XVII is expressed by neurons in the human brain, although its exact intracellular location and function have remained unknown.