Br J Cancer 2006,94(3):436–445.PubMedCrossRef Competing interests
The authors declare that they have no competing interests. Authors’ contributions YM carried out RT-PCR and Western blot, performed the statistical analysis and wrote the paper. LM participated in the design of the study and contributed with drafting the manuscript. QG carried out the immunohistochemistry studies. SLZ participated in coordination. All authors read and approved the final manuscript.”
“Background Animal models have been extremely critical in the understanding of cancer and in the pre-clinical selleck compound testing of new antitumor drugs since 1960s when it was first developed by implanting human colon carcinoma to nude mice . The utility of each particular model, nevertheless, depends on how close it replicates the original tumor. To the present days, several kinds of animals, like dog, monkey, and murine, have ever been tested and compared between each other
for the purpose of finding the best host for transplantation [2–4]. The results indicated that though the extent to which murine models recapitulate the features encountered in human tumor is still controversial, considering their reproducibility and availability, they still constitute a valuable in vivo system for the preclinical studies. Not surprisingly, an orthotopic model is much more superior to a heterotransplantation model in MK-2206 clinical trial that the former recapitulates the original tumor more likely. As far as human brain tumors are concerned, the orthotopic models currently available are established either by stereotaxic injection of cell suspensions [5–8] or implantation in solid piece through complicated craniotomy [9, 10]. Taking into consideration both the advantages
and disadvantages of the current methods, there is still much room for improvement. Recently, high success rate of model development of brain tumor were established using cell suspensions SB-3CT directly derived from fresh patient brain tumors indicating the important role of stromal cells in tumor formation . In the current study, we developed orthotopic xenograft mouse model by injecting tiny tumor tissue, but not cell suspensions, into the brain of mouse with a special trocar system. It is argued that the organ-specific microenvironment plays a determining role in the growth patterns of transplanted tumors [12, 13]. To observe the growth patterns of different tumor types implanted to the same organs, we chose primary glioblastoma multiforme and brain metastasis for transplantation in this study. The growth of xenografts in the mice brain was observed with MRI. Histological study was also performed to explore and compare the growth features of these two biologically distinctive malignances.