Late apoptosis was determined by TUNEL (DNAse-I) staining. Proliferation was assessed by Ki-67 and PCNA staining. HIF-1 alpha induction induces significantly higher PCNA/Ki-67 expression paralleled by bax/bcl-2 ratio < 1 favorable for anti-apoptotic conditions. By downregulation of CUX1 after HIF-1-alpha induction early and late apoptosis markers caspase 3/7 and their cleavage products and DNASe-I were highly significantly induced followed find more by significantly loss of PCNA and Ki-67 positive cells. CUX1 and its role in hcc has to be further investigated a potential new therapeutic target. Disclosures:
The following people have nothing to disclose: Carmen Rothmund, Pietro Di Fazio, Heidi Griesmann, Benjamin Kuehnemuth, Thomas Gress, Patrick Michl, Thaddaeus T. Wissniowski Background The role of alpha-feto protein (AFP) in the diagnosis of hepatocellular carcinoma (HCC) is getting smaller due to the advances of imaging modalities. However, consecutive increment of AFP level in liver cirrhosis patients is
presumed to be associated with the higher risk of developing HCC in clinical settings. Such a notion instigated us to analyze serial AFP levels of HCC patients in a retrospective manner. Methods From January 2002 to December 2012, 1931 patients were diagnosed with HCC in Seoul St. Mary’s hospital. Among them 133 patients were found to have a serial record of AFP measurements for over one year. We assessed AFP levels at the time the diagnosis of HCC was made and compared them with that of patients selleck screening library at 3,6 and 12 months prior to the diagnosis. Results At the time the diagnosis was made, the patients’ baseline characteristics were as follows; mean age was 58.24 years (32-87), median tumor size was 2.2cm (0.9-26.3), median AFP level was 45.53 ng/mL (1.4-32134). Median AFP level of 12 months, 6 months and 3 months before the diagnosis of HCC was 6.19 ng/mL (1.12-513), 7.53 ng/mL (0.96-1287.86), 11.94 ng/mL (0.91-1461), 45.53 ng/mL (1.4-23134), respectively. Consecutive increment of AFP level was statistically significant in time dependent
manner (p<0.000) with linear relationship (P=0.001). MCE公司 We divided patients by two groups; one was AFP over 45 ng/mL at the time of diagnosis of HCC, and the other one was not. In elevated AFP group (n=67), median AFP level of 12 months, 6 months and 3 months before the diagnosis of HCC and at the time of the diagnosis of HCC was 11.76 ng/mL (1.3513), 26.82 ng/mL (1.4-1287.86), 76.92 ng/mL (3-1461), 476 ng/mL (45.53-23134), respectively. In non-elevated AFP group (n=66), median AFP level was 5.37 ng/mL (1.1274.78), 6.09 ng/mL (0.96-91), 5.51 ng/mL (0.91-30.01), 5.63 ng/mL (1.4-40.1), respectively. In elevated AFP group, consecutive increment of AFP level was statistically significant in time dependent manner (p≤0.000). However, there was no significant change of consecutive AFP level In non-elevated AFP group.