Hu went, we assume that 53 IMD1 k Nnten Gamma-Secretase Inhibitors have antioxidant effects on heart muscle cells. We investigated the effect of antioxidant IMD1 53 Myokardsch The I / R in vivo induced in rats and their effects on the Lebensf Ability of rat cardiomyocytes and apoptosis by H2O2 induced in vitro treatment of cardiomyocytes rats and explained Utert underlying the way 53 signaling IMD1 exercise its protective effect. Second Materials and methods 2.1. Materials Sprague-Dawley male pattern day-old rats and 2 1 SD rats were obtained from the Animal Center, Health Science Center, Peking University t. All animal care and experimental protocols correspond to the rule of the Animal Management Department of Health, PeopleRepublic of China and the Guide for the Care and Use of Laboratory Animals by the U.S. National Institutes of VER Published health and was developed by the Animal Care Committee of the Health Sciences Center, Peking University t approved. Synthetic peptide rat was 53 IMD1 Phoenix Pharmaceutical Inc.. Antip47phox polyclonal rabbit, anti-extracellular Ren signal-regulated kinase 1/2, and polyclonal goat anti-gp91phox, anti-phospho ERK1 / 2 and anti-actin were from Santa Cruz Biotechnology. Kit for superoxide dismutase and catalase were from Nanjing Jiancheng Bioengineering Mn of caspase 3 in Beyotime Institute for Biotechnology and Hoechst-F Staining of Applygen Technologies. 3 2,5 diphenyltetrazolium bromide, sodium dodecyl sulfate and MEK inhibitor PD98059 were from Sigma. Other chemicals and reagents were of analytical quality t. 2.2. Myocardial I / R rat model m Nnliche SD rats were measured using an intraperitoneal injection of urethane. The trachea was intubated for artificial ventilation with room air. A cannula was inserted into the right carotid artery for blood pressure measurement. Thoracotomy was performed between the sternum and left Costa. The pericardium was GE Opened and the heart exposed. Suture 3/0 silk around the left anterior descending coronary artery and the coronary artery was performed by the seam closely confined. After 30 min Myokardisch Mie 120 min reperfusion, the suture was released. The animals underwent sham surgery, the same surgery, au That the seam he was descending coronary artery to the LAD is not set. Or normal saline Solution was infused min IMD1 53 from the left femoral vein 20 min after the start of Ish Chemistry for 10 min. A standard extremity Tenableitung II was for electrocardiographic monitoring may need during the used I / R. At the end of reperfusion, the rats were deeply in Sthesiert with urethane, and blood was collected in heparinized syringes from the left ventricle and in R Hrchen for lactate dehydrogenase, superoxide dismutase and Mn catalase test, malondialdehyde content in the plasma. All animals were bled by get Tet. 2.3. Production of prime Ren neonatal cardiomyocytes and neonatal rat cardiomyocytes H2O2 treatment were isolated from January to February dayold SD rats. Briefly, excised hearts washed Hanks Balanced Salt Solution, and ventricular Ren tissues were ground using a fine scissors in HBSS and digested with trypsin and collagenase at 37 . The cells were isolated by multiple rounds of 10 min of tissue digestion. After each incubation the supernatant to an equal volume of DMEM added words .
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