Burkholderia DBT1, a bacterial strain isolated from oil refinery

Burkholderia DBT1, a bacterial strain isolated from oil refinery drainage, has been shown to be capable of degrading DBT in liquid culture oxidatively, through the Kodama pathway, within 3 days of incubation (Di Gregorio et al., 2004). Because DBT behaves as a recalcitrant compound and tends to bioaccumulate throughout the food chains, the isolation and characterization of bacterial strains able to degrade condensed thiophenes, using them as the sole source of carbon and energy, can result in applications in bioremediation protocols. Nevertheless, for the harmless exploitation of Burkholderia DBT1 in environmental biotechnology, a probative exclusion

of this strain from the B. cepacia complex is a prerequisite. The versatile metabolism of DBT1 towards PAHs such as naphthalene, phenanthrene and fluorene shown in the present study is an

encouraging trait for the possible use of this strain PD-166866 in vivo in the clean-up of contaminated sites. Moreover, the taxonomic details gained in this study attribute the strain DBT1 to the species fungorum, excluding any possible association of this isolate to the Bcc. The authors thank the Academy of Finland (grant no. 118637) for support. “
“Two strains of aerobic acidophilic chemoorganotrophic selleck chemical bacteria designed strains AP8T and AP9 were isolated from acid mine drainage and acidic soil, respectively. These isolates were Gram-negative, nonmotile cocci and coccobacilli measuring 0.5–0.8 μm in diameter. Cells were capsulated. Colonies on solid media were pink colored. The pH range for growth was 3.0–6.0 (optimum pH 4.5). Sugars, gluconate, and some amino acids were good carbon and energy sources for growth. The main components of cellular fatty acids were C15:0 iso and C16:1ω7c. Menaquinone-8 was the major quinone. The G+C content of genomic DNA was 59.5%. Both strains had identical sequences of 16S rRNA genes that were most closely related to that of the type strain of Acidobacterium capsulatum (96% similarity). There were major differences between the isolates and A. capsulatum in cell morphology, carbon nutrition, and fatty acid profiles. Based on these phylogenetic and phenotypic data, we propose the name Acidipila

rosea gen. nov., sp. nov. to accommodate Amino acid the novel isolates. The type strain is AP8T (NBRC 107607T, KCTC 23427T). Culture-independent molecular approaches have revealed the widespread occurrence of members of the phylum ‘Acidobacteria’ in nature. Large numbers of 16S rRNA gene clones of this phylum have been retrieved from soils (Janssen, 2006; Otsuka et al., 2008; Kenzaka et al., 2010), sediments (Dunbar et al., 1999; Barns et al., 2007), wastewater (LaPara et al., 2000; Narihiro et al., 2009), acid mine drainage (AMD) (Diaby et al., 2007; Tan et al., 2007), and hot springs (Hobel et al., 2005). The biodiversity of the Acidobacteria is potentially as great as that of the phylum Proteobacteria (Ludwig et al., 1997; Hugenholtz et al., 1998). Barns et al.

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