Based on the firing
rates of MUA versus single units, we estimate that a typical MUA contained 10 to 20 single units, thereby providing a reasonable local average. Henceforth, we refer to these unsorted MUAs with the SUA excluded as the same-site MUAs. Same-site MUA PPCs did not differ between sites delivering isolated NS versus BS units (Figure 1E) (not significant [n.s.], bootstrap test). This suggests that the overall gamma locking did not differ between the recording locations of BS and NS cells. Please note that if the MUA from a BS or NS recording site had been biased to contain more BS or NS cells, this would have created similar differences for the same-site MUA as for the respective SUA analysis, which we did this website not find. Although the same-site MUA PPC did not differ between NS and BS cells, it is conceivable that same-site MUA PPC varied across sites. In order to eliminate the variability
in PPCs across units Selleckchem VX-809 that is caused by differences in recording location, we computed, for each unit separately, the SUA-MUA PPC difference. This measure is defined as the difference between a SUA’s PPC and its corresponding same-site MUA’s PPC [PPCSUA – PPCMUA], such that a value >0 indicates stronger spike-LFP locking for the SUA than its corresponding same-site MUA. SUA-MUA gamma PPC difference was higher for NS than BS cells (p < 0.05, randomization test) and significantly different from zero only for NS cells (p < 0.05, bootstrap test) (Figure 1F). Hence, it is unlikely that the observed difference in gamma PPC between NS and BS cells (Figure 1D) was caused by differences in recording locations. In neocortex, there are more BS than NS cells (Figure 1B, Mitchell et al. (2007)). However, NS cells have higher firing rates, such that the MUA may contain approximately equal proportions of NS and BS spikes. Based on these estimates, the MUA-LFP PPC is expected to attain PPC values in between
the BS and NS cells’ PPC. In addition, Edoxaban we will demonstrate below that BS and NS cells lock on average to different gamma phases and that individual single units often lock to widely varying gamma phases. Assuming that our MUAs typically contained both BS and NS cells and individual cells that cover at least a small part of the overall intercell phase variance, this predicts that the MUA-LFP PPC is substantially smaller than the average PPC of its constituent SUAs, consistent with our observations. We found that NS cells were more gamma locked than BS cells during the sustained visual stimulation period. NS cells might also be more gamma locked than BS cells during network states in which cells receive only weak excitatory drive. Previous studies have shown that MUA-LFP gamma locking and LFP gamma power are weak in the absence of visual stimulation or in the presence of low-contrast visual stimuli in the RF (receptive field) (Fries et al.