Genome properties The genome of Anaerococcus pacaensis strain 9403502T is estimated at 2.36 Mb long with a G+C content of 35.05% (Figure 6 and Table 3). A total of 2,186 protein-coding and 72 RNA genes, including 3 rRNA genes, 42 tRNA, 1 tmRNA selleck Tubacin and 26 miscellaneous other RNA were founded. The majority of the protein-coding genes were assigned a putative function (74.1%) while the remaining ones were annotated as hypothetical proteins. The properties and the statistics of the genome are summarized in Tables 3 and and4.4. The Table 5 presents the difference of gene number (in percentage) related to each COG categories between Anaerococcus pacaensis and Anaerococcus prevotii DSM 20548. The proportion of COG is highly similar between the two species.
The maximum difference is related to the COG “Carbohydrate Metabolism and transportation” which does not exceed 1.94%. The distribution of genes into COGs functional categories Inhibitors,Modulators,Libraries is presented in Table 6. Figure 6 Graphical circular map of the genome. From outside to the center: scaffolds are in grey (unordered), genes on forward strand (colored by COG categories), genes on reverse strand (colored by COG categories), RNA genes (tRNAs green, rRNAs red, tm RNAs black, … Table 3 Project information Table 4 Nucleotide content and gene count levels of the genome Table 5 Number of genes associated with the 25 general COG functional categories Table 6 Percentage of genes associated Inhibitors,Modulators,Libraries with the 25 general Inhibitors,Modulators,Libraries COG functional categories for Anaerococcus pacaensis and Anaerococcus prevotii DSM 20548.
Insights into the genome sequence We made some brief comparisons against Anaerococcus prevotii DSM 20548 (“type”:”entrez-nucleotide”,”attrs”:”text”:”NC_013171″,”term_id”:”257065520″,”term_text”:”NC_013171″NC_013171), Inhibitors,Modulators,Libraries which is currently the closest available genome. This genome contains 1 chromosome (accession number: “type”:”entrez-nucleotide”,”attrs”:”text”:”NC_013171″,”term_id”:”257065520″,”term_text”:”NC_013171″NC_013171) and 1 plasmid (accession number: “type”:”entrez-nucleotide”,”attrs”:”text”:”NC_013164″,”term_id”:”256821123″,”term_text”:”NC_013164″NC_013164). The draft genome sequence of Anaerococcus pacaensis has a bigger size compared to the Anaerococcus prevotii (respectively 2,36 Mbp and 1,99 Mbp). The G+C content is slightly larger than Anaerococcus prevotii too (respectively 37.5% and 35.05%).
Anaerococcus pacaensis shares more genes (2,272 genes against 1,916 genes), however Inhibitors,Modulators,Libraries the ratios Carfilzomib of genes per Mb is very similar (962,71 �C 962,81). Conclusion On the basis of phenotypic, phylogenetic and genomic analysis, we formally propose the creation of Anaerococcus pacaensis, whichcontains the strain 9403502T. This bacterium has been found in Marseille, France. Description of Anaerococcus pacaensis sp. nov. Anaerococcus pacaensis (pa.ca��en.sis L. gen. masc. n.